cdk5 p35  (Tanabe)

Journal: bioRxiv

Article Title: Curcumin attenuates microglia-mediated chronic neuropathic pain through CDK5 /p35 signaling pathway

doi: 10.1101/2024.11.13.623498

Figure Lengend Snippet: (A) Experimental protocol for CCI or Sham surgery, behavioral test, injection of curcumin and collection of bilateral spinal cord in the Sham+DMSO group, the CCI+DMSO group and the CCI+Cur group. (B, D) Representative images showed WB analysis of p35 expression in ipsilateral and contralateral spinal cord. (C, E) Results showed the effects of i.p. injection of curcumin on the p35 expression in the ipsilateral and contralateral spinal cord. (F, H) Representative images showed WB analysis of CDK5 expression in ipsilateral and contralateral spinal cord. (G, I) Results showed the effects of i.p. injection of curcumin on the CDK5 expression in the ipsilateral and contralateral spinal cord. (J) Experimental protocol for CCI or Sham surgery, behavioral test, injection of curcumin and collection of bilateral spinal cord in the CCI+DMSO group, the CCI+Cur30 group, the CCI+Cur100 group and the CCI+Cur300 group. (K, M) Representative images showed WB analysis of p35 expression in ipsilateral and contralateral spinal cord. (L, N) Results showed the effects of i.p. injection of curcumin with different doses on the p35 expression in the ipsilateral and contralateral spinal cord. (O, Q) Representative images showed WB analysis of CDK5 expression in ipsilateral and contralateral spinal cord. (P, R) Results showed the effects of i.p. injection of curcumin with different doses on the CDK5 expression in the ipsilateral and contralateral spinal cord. **** P < 0.0001, ** P < 0.01, * P < 0.05, n=6/group ; CCI: chronic compression injury; Cur: curcumin; WB: western blotting; i.p.: intraperitoneal injection; CDK5: Cyclin-dependent kinase 5

Article Snippet: The sections were blocked with 10% sheep serum for 2 h at room temperature and incubated with the following primary antibodies for 48 h at 4 °C: goat-anti-Iba1 (1:100, Abcam), rabbit anti-p35(1:400, Cell Signaling Technology), rabbit anti-CDK5 (1:500, Cell Signaling Technology).

Techniques: Injection, Expressing, Western Blot

Journal: bioRxiv

Article Title: Curcumin attenuates microglia-mediated chronic neuropathic pain through CDK5 /p35 signaling pathway

doi: 10.1101/2024.11.13.623498

Figure Lengend Snippet: (A) Representative images of staining for p35, Iba-1, and DAPI in the ipsilateral spinal cord in the Sham+DMSO group, the CCI+DMSO group and the CCI+Cur group.. (B) Representative images of staining for CDK5, Iba-1, and DAPI in the ipsilateral spinal cord in the Sham+DMSO group, the CCI+DMSO group and the CCI+Cur group. (C) Results showed the effects of i.p. injection of curcumin on the co-expression of p35 and Iba-1 in the ipsilateral spinal cord. (D) Results showed the effects of i.p. injection of curcumin on the co-expression of CDK5 and Iba-1 in the ipsilateral spinal cord. (E) Schematic diagram of the co-expression of microglia marker Iba-1 and p35 or CDK5 in the ipsilateral spinal cord. **** P < 0.0001, *** P < 0.001, ** P < 0.01,n=6/group ; CCI: chronic compression injury; Cur: curcumin; i.p.: intraperitoneal injection; CDK5: Cyclin-dependent kinase 5

Article Snippet: The sections were blocked with 10% sheep serum for 2 h at room temperature and incubated with the following primary antibodies for 48 h at 4 °C: goat-anti-Iba1 (1:100, Abcam), rabbit anti-p35(1:400, Cell Signaling Technology), rabbit anti-CDK5 (1:500, Cell Signaling Technology).

Techniques: Staining, Injection, Expressing, Marker

Journal: bioRxiv

Article Title: Curcumin attenuates microglia-mediated chronic neuropathic pain through CDK5 /p35 signaling pathway

doi: 10.1101/2024.11.13.623498

Figure Lengend Snippet: (A) Experimental protocol for CCI or Sham surgery, behavioral test, injection of curcumin and collection of bilateral L4-6 DRG in the Sham+DMSO group, the CCI+DMSO group and the CCI+Cur group. (B, D) Representative images showed WB analysis of p35 expression in ipsilateral and contralateral DRG. (C, E) Results showed the effects of i.p. injection of curcumin on the p35 expression in the ipsilateral and contralateral DRG. (F, H) Representative images showed WB analysis of CDK5 expression in ipsilateral and contralateral DRG. (G, I) Results showed the effects of i.p. injection of curcumin on the CDK5 expression in the ipsilateral and contralateral DRG. (J) Experimental protocol for CCI or Sham surgery, behavioral test, injection of curcumin and collection of bilateral L4-6 DRG in the CCI+DMSO group, the CCI+Cur30 group, the CCI+Cur100 group and the CCI+Cur300 group. (K, M) Representative images showed WB analysis of p35 expression in ipsilateral and contralateral DRG. (L, N) Results showed the effects of i.p. injection of curcumin with different doses on the p35 expression in the ipsilateral and contralateral DRG. (O, Q) Representative images showed WB analysis of CDK5 expression in ipsilateral and contralateral DRG. (P, R) Results showed the effects of i.p. injection of curcumin with different doses on the CDK5 expression in the ipsilateral and contralateral DRG. *** P < 0.001, ** P < 0.01, * P < 0.05, n=6/group; CCI: chronic compression injury; DRG: dorsal root ganglia; Cur: curcumin; WB: western blotting; i.p.: intraperitoneal injection; CDK5: Cyclin-dependent kinase 5

Article Snippet: The sections were blocked with 10% sheep serum for 2 h at room temperature and incubated with the following primary antibodies for 48 h at 4 °C: goat-anti-Iba1 (1:100, Abcam), rabbit anti-p35(1:400, Cell Signaling Technology), rabbit anti-CDK5 (1:500, Cell Signaling Technology).

Techniques: Injection, Expressing, Western Blot

Journal: bioRxiv

Article Title: Curcumin attenuates microglia-mediated chronic neuropathic pain through CDK5 /p35 signaling pathway

doi: 10.1101/2024.11.13.623498

Figure Lengend Snippet: (A) Representative images of staining for p35, Iba-1, and DAPI in the ipsilateral L4-6 DRG in the Sham+DMSO group, the CCI+DMSO group and the CCI+Cur group. (B) Representative images of staining for CDK5, Iba-1, and DAPI in the ipsilateral DRG in the Sham+DMSO group, the CCI+DMSO group and the CCI+Cur group. (C) Results showed the effects of i.p. injection of curcumin on the co-expression of p35 and Iba-1 in the ipsilateral DRG. (D) Results showed the effects of i.p. injection of curcumin on the co-expression of CDK5 and Iba-1 in the ipsilateral DRG. (E) Schematic diagram of the co-expression of microglia marker Iba-1 and p35 or CDK5 in the ipsilateral DRG. **** P < 0.0001, *** P < 0.001,n=6/group ; DRG: dorsal root ganglia; CCI: chronic compression injury; Cur: curcumin; i.p.: intraperitoneal injection; CDK5: Cyclin-dependent kinase 5

Article Snippet: The sections were blocked with 10% sheep serum for 2 h at room temperature and incubated with the following primary antibodies for 48 h at 4 °C: goat-anti-Iba1 (1:100, Abcam), rabbit anti-p35(1:400, Cell Signaling Technology), rabbit anti-CDK5 (1:500, Cell Signaling Technology).

Techniques: Staining, Injection, Expressing, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Timing dependent neuronal migration is regulated by Cdk5-mediated phosphorylation of JIP1

doi: 10.3389/fcell.2024.1371568

Figure Lengend Snippet: The relationship between expression of Cdk5, p35 and JIP1 across cerebral cortex cells. (A–D) Low-dimensional representation of single cells from mouse cerebral cortex, based on tSNE embedding of single-cell RNA-seq data 15. Cells are colored based on (A) the cell types and animal age, which ranges from embryonic E12 to E18, (B) Cdk5 expression, (C) Cdk5r1 (p35) expression, (D) Mapk8ip1 (JIP1) expression, (E) Pseudotime analysis of Cdk5, Cdk5r1 and Mapk8ip1 dynamic expression in Intermediate progenitors. (F) Expression of Cdk5, Cdk5r1 and Mapk8ip1 at different embryonic development ages. (G) Expression of Cdk5, p35 and JIP1 in embryonic brains was analyzed by Western blotting.

Article Snippet: Mouse anti-JIP1 (B-7, Cat. #sc-25267), Rabbit anti-p35 (C-19, Cat. #sc-820), Rabbit anti-Cdk5 Antibody (C-8, Cat. # sc-173) were purchased from Santa Cruz Biotechnology (Texas, United States).

Techniques: Expressing, RNA Sequencing, Western Blot